Question

The forward primer was provided by the manufacturer as a lyophilized powder. If the manufacturer delivers 127.4 nmoles of this primer, what volume of buffer should you dissolve it in to make a 100 μM stock?

Answer 1

Assuming the buffer has a density of 1 g/mL, the volume of buffer needed to dissolve 127.4 nmoles of the primer in order to make a 100 μM stock would be 127.4 μL.