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To calculate the protein concentration, you need to know the purity of the protein and its molecular weight. Here's how you can calculate it:
1. Convert the purity percentage to a decimal by dividing it by 100:
Purity = 97.3% = 97.3/100 = 0.973
2. Calculate the effective concentration by multiplying the purity by 100:
Effective Concentration = Purity x MW
= 0.973 x 2666.31 kDa
Make sure the units are consistent. In this case, the purity is a decimal, and the molecular weight is given in kDa (kilodaltons).
The effective concentration will be in units of kDa. If you want to convert it to a different unit (e.g., mg/mL), you'll need additional information, such as the volume or mass of the protein solution.