Question

The following DNA sequence represents a eukaryotic gene. Indicate which is the template and which is the coding strand, determine where transcription begins (assume it ends at the end of the sequence presented), write out the nucleotide sequence of the initial transcript and the processed mRNA, indicating and removing any introns in the process. Finally, translate the mRNA and write out the amino acid sequence of the encoded polypeptide.


5’-GGCGATATAAAGGCGTATGACAGATGATGTTCACCCCCTTGGGAGATGTTCCTCACCAAAGAGAAGCCGGTGAGTATTACACAATACTTATCCGGAGTG...
3’-CCGCTATATTTCCTCATACTGTCTACTACAAGTGGGGGAACCCTCTACAAGGAGTGGTTTCTCTTCGGCCAGTCATAATGTGTTATGAATAGGCCTCAC...


...CCTTCCCTTTCTGAGGGGACCTTTTCGTGCACGAGCTAATTTTGAAGTCCCAATAAACGCCGTACTGTGTGTGAGAGAGAGGCCGGGGGCCCTTGCC-3’
...GGAAGGGAAAGACTCCCCTGGAAAAGCACGTGCTCGATTAAAACTTCAGGGTTCTTTGCGGCATGACACACACTCTCTCTCCGGCCCCCGGGAACGG-5’

Answer 1

A fragment of mRNA with the sequence 5' ACGCAUGGCAAAAAAAA 3' will have a template DNA sequence that reads
A. 3' UGCGUACCGUUUUUUUU 5'.
B. 3' TTTTTTTTGCCATGCGT 5'.
C. 3' UUUUUUUUGCCATGCGT 5'.
D. 3' TGCGTACCGTTTTTTTT 5'.

Answer 2

3'TGCGTACCGTTTTTTTT 5'