Question
In a thin layer chromatography (TLC)experiment,the TLC plate was pre-developed in the development chamber containing the mobile phase (to remove any contaminants before use),then air dried.
On the same day,the TLC plate was then spotted with the sample & standard and placed in the same development chamber containing the mobile phase. Is this acceptable or should the development chamber be replaced with fresh mobile phase?
On the same day,the TLC plate was then spotted with the sample & standard and placed in the same development chamber containing the mobile phase. Is this acceptable or should the development chamber be replaced with fresh mobile phase?
Answers
DrBob222
I don't know the text book answer but I think the mobile phase should be replaced.