Yes, you need a comma after "book." That's an introductory clause up to that point. See #3 here:
http://grammar.ccc.commnet.edu/grammar/commas.htm
The sentence sounds fine except for "one's" -- who is that "one"?
Do I need a comma after the word book and does the sentence sound alright?
When I first saw the title of the book I knew the theme would pertain to the importance of one’s identity.
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