Asked by tech

when i do imaging of tissue to look at collagen structure using a 2 photon microscopy, but im not familiarized with the methods to measure the diameter of the collagen and the area occupied by it. Does anyone know how do researchers measure them
Answered by drwls
I am not familiar with the technique and had to look it up here:
http://en.wikipedia.org/wiki/Two-photon_excitation_microscopy

The device has a scanning mirror. It should be precalibrated for spatial scale. Check the instruction manual for the spatial field of view at various magnification settings
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