Question
You have 20mL of a solution of concentration 0.015mg/mL of x in test tube #1.
Prepare serial dilutions of your stock solution, and calculated the concentration of x as follows:
Add 10mL of distilled water and 10mL of stock solution from test tube 1 to test tube 2.
Add 10mL of distilled water and 10mL of stock solution from test tube 2 to test tube 3.
.....and it keeps going.
So, obviously I will use the formula c1v1=c2v2.
So the concentration of test tube #1 is just 0.015mg/mL.
So, for test tube #2, c1=0.015mg/mL, and v1=20mL, and c2 is what we are trying to solve for. But what is v2? Can someone please tell me what v2 is and explain what it is. I think it's 40mL, giving c2=0.00750 mg/mL. But can someone please explain how I know what volumes to add! And maybe even explain what v2 for the next test tube would be? Is it 80mL?
Prepare serial dilutions of your stock solution, and calculated the concentration of x as follows:
Add 10mL of distilled water and 10mL of stock solution from test tube 1 to test tube 2.
Add 10mL of distilled water and 10mL of stock solution from test tube 2 to test tube 3.
.....and it keeps going.
So, obviously I will use the formula c1v1=c2v2.
So the concentration of test tube #1 is just 0.015mg/mL.
So, for test tube #2, c1=0.015mg/mL, and v1=20mL, and c2 is what we are trying to solve for. But what is v2? Can someone please tell me what v2 is and explain what it is. I think it's 40mL, giving c2=0.00750 mg/mL. But can someone please explain how I know what volumes to add! And maybe even explain what v2 for the next test tube would be? Is it 80mL?
Answers
never mind, i got it :)
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