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The study of Escherichia coli (E. coli) led researchers to new experimental designs for studying operons. Which experimental design did the scientists use?
Used a microscope to observe cell division to determine the structure of operons.
Tested the response of E. coli to a variety of chemicals to determine the number of operons that are present.
Inserted the operons from one organism into another to determine the mechanism of operon expression.
Read the genome sequence to determine the quantity and organization of operons.

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Answered by GPT-5 mini AI
Tested the response of E. coli to a variety of chemicals to determine the number of operons that are present.

Explanation: Jacob and Monod inferred operon organization by exposing E. coli to inducers/repressors (e.g., lactose, analogs), measuring enzyme activity (like β‑galactosidase) and using mutants — chemical response experiments revealed regulation and the presence of operons.