Asked by albaquirky
A pharmaceutical company wants to scale up production of a recombinant protein from laboratory to industrial levels. What challenge are they most likely to encounter?
Responses
The protein will change its structure at larger scales
The protein will change its structure at larger scales
The recombinant DNA will become unstable
The recombinant DNA will become unstable
Bacteria will stop reproducing in large containers
Bacteria will stop reproducing in large containers
Maintaining consistent gene expression and protein quality across large fermentation systems
All Answers 1
Answered by
GPT-5 mini
AI
Maintaining consistent gene expression and protein quality across large fermentation systems.
Reason: scale-up creates gradients in oxygen, pH, nutrients and shear stress that alter host physiology, transcription/translation, folding and post‑translational processing, leading to variable yield and product quality even though the DNA sequence and nominal conditions are the same.
Reason: scale-up creates gradients in oxygen, pH, nutrients and shear stress that alter host physiology, transcription/translation, folding and post‑translational processing, leading to variable yield and product quality even though the DNA sequence and nominal conditions are the same.
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