Question

Why might a scientist choose to use multiple restriction enzymes on the same DNA sample?
Responses

To get more detailed information about the DNA structure
To get more detailed information about the DNA structure

To speed up the cutting process
To speed up the cutting process

To reduce the cost of the experiment
To reduce the cost of the experiment

To make the DNA more stable

Answer 1

Correct answer: To get more detailed information about the DNA structure.

Why: Different restriction enzymes cut at different recognition sequences, producing different fragment patterns. Using several enzymes (alone or in combination) gives higher-resolution restriction maps, helps confirm sequence identity, locate features, and create specific ends for cloning. The other choices are incorrect: using more enzymes usually doesn't speed cutting, reduce cost, or make DNA more stable.