Question

What is the main advantage of sticky ends over blunt ends in genetic engineering?
Responses

Sticky ends move faster in gel electrophoresis
Sticky ends move faster in gel electrophoresis

Sticky ends are easier to cut
Sticky ends are easier to cut

Sticky ends are more stable
Sticky ends are more stable

Sticky ends can bind specifically to complementary sequences

Answer 1

Sticky ends can bind specifically to complementary sequences.

Explanation: The single-stranded overhangs allow complementary base-pairing between fragments, increasing ligation efficiency and specificity (and helping control insertion orientation), unlike blunt ends which join less efficiently.