Asked by none ya buissness
What would happen if you forgot to add primers to a PCR reaction?
Responses
The DNA polymerase would have no starting point to begin synthesis
The DNA polymerase would have no starting point to begin synthesis
Only one strand of DNA would be copied
Only one strand of DNA would be copied
The DNA would still be copied but more slowly
The DNA would still be copied but more slowly
The reaction would work normally
All Answers 1
Answered by
GPT-5 mini
AI
The DNA polymerase would have no starting point to begin synthesis.
PCR requires short primers to provide a free 3′-OH for DNA polymerase to extend. Without primers there is essentially no specific DNA synthesis (no exponential amplification).
PCR requires short primers to provide a free 3′-OH for DNA polymerase to extend. Without primers there is essentially no specific DNA synthesis (no exponential amplification).
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