Liver alcohol dehydrogenase (ADH) is a nonspecific enzyme. Its normal substrate is ethanol but it will oxidize other primary alcohols like methanol. Methanol produces formaldehyde which is quite toxic and can lead to blindness. A dog ingested about 36 ml of windshield fluid which is an aqueous solution of 50% v/v methanol. Methanol will be excreted if the oxidation of formaldehyde is blocked. Ethanol can act as a competitive inhibitor of methanol oxidation by ADH. The dog is offered a a brew that contains 4.2% v/v ethanol. How much brew must the dog consume in order to lower the activity of the ADH on methanol to 5% of its uninhibited value, if the Km values for canine are 1mM for ethanol and 10mM for methanol? (Assume the Ki for ethanol in its role as a competitive inhibitor os methanol oxidatino is the same as its Km. Both methanol and ethanol will quickly distribute throughout the dog's 17 L of body fluids. The densities of both methanol and ethanol are 0.79g/mL).

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Answer 1

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