Asked by kj
Scientists hypothesized that E. coli contained one operon. After further experimentation, they discovered that, in fact, E. coli contained two operons. Using this information, answer the questions provided
What is one way scientists are able to study operons in organisms?
by studying the number and organization of operons by reading the genome sequence
by counting the operons during cell division
by studying the operon controls during translation when proteins are constructed
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Correct choice: by studying the number and organization of operons by reading the genome sequence.
Why: operons are DNA arrangements (clusters of co‑transcribed genes plus their regulatory sequences). Genome sequencing and bioinformatics let scientists find adjacent genes with shared promoters/operators and predict operon structure (confirmed experimentally with techniques like RT‑PCR, Northern blots, reporter fusions). Counting during cell division or focusing only on translation would not reveal operon organization.
Why: operons are DNA arrangements (clusters of co‑transcribed genes plus their regulatory sequences). Genome sequencing and bioinformatics let scientists find adjacent genes with shared promoters/operators and predict operon structure (confirmed experimentally with techniques like RT‑PCR, Northern blots, reporter fusions). Counting during cell division or focusing only on translation would not reveal operon organization.
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