Question

Question 1
What is CRISPR?

a
A double stranded RNA virus
b
A gene editing tool
c
Delicious potato chips
d
A phospholipid
Question 2
CRISPR can use a specific RNA guide can be made in a lab o match he desired gene to be edited.

True
False


How DNA Fingerprinting Actually Works

Question 3
Steps of DNA Fingerprinting
Put the following steps of DNA fingerprinting in the correct order, from first to last.

Reorder answers
1.Gel electrophoresis separates DNA fragments based on size.
Reorder answers
2.The DNA pattern is visualized as bands that can be compared between samples.
Reorder answers
3.DNA fragments are transferred to a nylon membrane and exposed to radioactive probes.
Reorder answers
4.DNA is extracted from cells.
Reorder answers
5.DNA is cut into fragments using restriction enzymes.
Question 4
Which of the following is an application (use) of DNA Fingerprinting?

a
It is used to solve crimes where DNA has been left behind.
b
To identify remains of victims
c
To determine familial relationships (who's the baby daddy)
d
All of these are uses for DNA fingerprinting
Question 5
DNA Band Pattern Diagram (image of a gel)
Lane Source Band Pattern (from top to bottom/horizontally)
1 Crime Scene Sample █  █ █   █ █
2 Suspect A █ █   █ █  █
3 Suspect B █  █ █   █ █
4 Suspect C █ █  █ █ █   
(Each "█" represents a DNA band. The positions indicate fragment sizes. Each color represents one sample.)

Which suspect, if any, left their DNA at the crime scene?

a
Suspect A
b
Suspect B
c
Suspect C
d
No matching samples
PCR - The Steps of Polymerase Chain Reaction

Polymerase Chain Reaction (PCR)

Question 6
What are the main steps of PCR?

Reorder answers
1.Denaturation of the DNA at 94 to 98 oC
Reorder answers
2.Primer extension at 72 oC
Reorder answers
3.Primer annealing at 55 to 65 oC
Reorder answers
4.After one cycle, DNA molecules are doubled
Question 7
The main role of PCR is to quickly make many copies of the same segment of DNA.

True
False


How Does Cloning Work?



Click and Clone Mimi interactive

Question 8
What is used to create human insulin by bacteria?

a
CRISPR
b
Cloning
c
Restriction enzymes
d
Recombinant DNA
Question 9
When a clone is created, the clone is genetically __________ the parent.

a
very slightly different
b
exactly like
c
different than
d
similar to
Question 10
Directions: Match each biotechnology tool or process (Column A) with its correct description or application (Column B).

Each answer will be used only once.

Column A
1.
Gel electrophoresis:
Gel electrophoresis
2.
Recombinant DNA:
Recombinant DNA
3.
PCR (Polymerase Chain Reaction):
PCR (Polymerase Chain Reaction)
4.
Gene therapy:
Gene therapy
5.
DNA fingerprinting:
DNA fingerprinting
Column B
a.Used to separate DNA fragments by size and charge
b.Replacing a defective gene with a normal one to treat disease
c.Process used to make many copies of a specific DNA segment
d.Inserting a gene from one organism into another organism’s DNA
e.Identifies individuals based on their unique DNA banding patterns

Answer 1

Question 1
b. A gene editing tool

Question 2
True

Question 3 (correct order)
4. DNA is extracted from cells.
5. DNA is cut into fragments using restriction enzymes.
1. Gel electrophoresis separates DNA fragments based on size.
3. DNA fragments are transferred to a nylon membrane and exposed to radioactive probes.
2. The DNA pattern is visualized as bands that can be compared between samples.

Question 4
d. All of these are uses for DNA fingerprinting

Question 5
b. Suspect B

Question 6 (correct order)
1. Denaturation of the DNA at 94 to 98 °C
3. Primer annealing at 55 to 65 °C
2. Primer extension at 72 °C
4. After one cycle, DNA molecules are doubled

Question 7
True

Question 8
d. Recombinant DNA

Question 9
b. exactly like

Question 10 (matches)
1. Gel electrophoresis: a. Used to separate DNA fragments by size and charge
2. Recombinant DNA: d. Inserting a gene from one organism into another organism’s DNA
3. PCR (Polymerase Chain Reaction): c. Process used to make many copies of a specific DNA segment
4. Gene therapy: b. Replacing a defective gene with a normal one to treat disease
5. DNA fingerprinting: e. Identifies individuals based on their unique DNA banding patterns