Question
The temperature and duration at which Media Fill samples must be incubated are:
A. 20-25 degrees Celsius for no less than 48 then 30-35 degrees Celsius for no less than 5 additional days
B. 30-35 degrees Celsius for no less than 48 hours then 20-25 degrees Celsius for no less than 5 additional days
C. 20-25 degrees Celsius for 3 to 5 days
D. 30-35 degrees Celsius for 3 to 5 days
E. 20-25 degrees Celsius for 7 days followed by 30-35 degrees Celsius for 7 days (or reverse order depending on the facility's SOP)
F. 20-25 degrees Celsius for 14 days followed by 30-35 degrees Celsius for 14 days (or reverse order depending on the facility's SOP)
A. 20-25 degrees Celsius for no less than 48 then 30-35 degrees Celsius for no less than 5 additional days
B. 30-35 degrees Celsius for no less than 48 hours then 20-25 degrees Celsius for no less than 5 additional days
C. 20-25 degrees Celsius for 3 to 5 days
D. 30-35 degrees Celsius for 3 to 5 days
E. 20-25 degrees Celsius for 7 days followed by 30-35 degrees Celsius for 7 days (or reverse order depending on the facility's SOP)
F. 20-25 degrees Celsius for 14 days followed by 30-35 degrees Celsius for 14 days (or reverse order depending on the facility's SOP)
Answers
GPT-5 mini
E. Media fill samples are incubated for a total of 14 days (typically 7 days at 20–25 °C followed by 7 days at 30–35 °C, or the reverse per facility SOP).