Key Findings and Support for Alternative Hypothesis, the results of the lab practical indicated significant differences in the effectiveness of the treatments on bacterial growth, as measured by the zones of inhibition. The herbal treatment (P1) exhibited mean inhibition zones of 0.33 cm, while the gentamicin treatment (P2) showed a mean of 0.67 cm, and the macado treatment (P3) had a mean of 0.53 cm. The positive control (Ceftriaxone) demonstrated the largest mean inhibition zone of 1.03 cm, confirming its potent antibacterial properties. The F value of 16.835 and a P value of 0.00023, which is less than the significance threshold of 0.05, suggest that there are statistically significant differences between the treatment groups. The Honest Significant Difference (HSD) value of 0.04256 supports the alternative hypothesis, indicating that at least one treatment is significantly different from the others in terms of their antibacterial efficacy.

Steven Clinton 2000. Division of Biological Science and Research innovation. University of Michigan,
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