In the late 1800s, Charles Darwin and his son Francis investigated phototropism, the growth of plants toward light. They discovered that a grass seedling, protected by a sheath called a coleoptile, could only bend toward light if the tip was intact. If the tip was removed or covered with an opaque cap, the coleoptile failed to bend. This led them to conclude that the tip of the coleoptile was responsible for sensing light and transmitting a signal down to the area below, where growth occurs.
Later, Danish scientist Peter Boysen-Jensen conducted further experiments to explore the nature of this signal. He used gelatin to separate the coleoptile tip from the rest of the plant, allowing chemical signaling but preventing direct contact. The seedlings still bent toward light, indicating that a chemical substance was responsible for the signaling. However, when he used an impermeable barrier (mica) to separate the tip, the phototropic response did not occur, reinforcing the idea that the signal was a mobile chemical that needed to reach the elongating region below the tip to initiate growth toward light.