To support the claim that mitochondria of the cell uses more glucose than fatty acids to convert to ATP, the most relevant evidence would come from analyzing the amount of ATP produced by mitochondria when processing each substrate separately. Therefore, the best option is:
Measure the amount of ATP produced by a mitochondria for glucose and compare it to the amount of ATP produced by a mitochondria for a fatty acid.
This approach directly assesses the efficiency and output of ATP production from each macronutrient, allowing for a straightforward comparison of their contributions to cellular energy production. The other options may provide useful information but are not as directly related to the core claim regarding ATP generation from glucose vs. fatty acids.