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Part A: Sample Preparation

Live plant material is used in the absorption analysis. Chlorophyll and other pigments are removed using the protocol provided below.
Materials (per team):
Balance
Coleus leaf
Mortar and pestle
30 mL of ethanol
35 mL syringe
100 mL beaker
Funnel lined with a coffee filter
Safety goggles
SAFETY
Wear goggles and aprons when working with chemicals.
Alcohol is volatile and flammable.
Avoid breathing in vapors of the reagents.
Procedure:
1. Prepare an extract of chlorophyll by weighing 0.3 grams of your leaf sample. Make sure the sample includes all of the different colors on the leaf. Tear the leaf section into small pieces and place the tissue in a mortar for grinding.
2. Add 10 mL of ethanol to the sample and grind with the pestle until only tiny specks of leaf tissue remain. Add an additional 20 mL of ethanol to the mortar.
3. Filter this liquid into a beaker through a funnel lined with a coffee filter.
4. Dispose of the filter in the designated trash bin.
use the above information and write a good procedure in a practical correctly in a sentence form

1 answer

Procedure for Chlorophyll Extraction:

  1. Begin by weighing out 0.3 grams of a Coleus leaf sample, ensuring that it includes the various colors present on the leaf. Tear the leaf into small pieces and transfer it to a mortar.

  2. Add 10 mL of ethanol to the leaf pieces in the mortar and use a pestle to grind the mixture until only tiny specks of leaf tissue remain visible.

  3. Once the grinding is complete, add an additional 20 mL of ethanol to the mixture in the mortar, continuing to grind to ensure thorough extraction of pigments.

  4. Carefully pour the resulting liquid into a 100 mL beaker, using a funnel lined with a coffee filter to filter out the solid leaf tissue.

  5. After filtration is complete, dispose of the used coffee filter in the designated trash bin, ensuring that all waste is handled according to safety guidelines.

  6. Finally, make sure to wear safety goggles and an apron throughout the procedure, and avoid breathing in any vapors, as alcohol is volatile and flammable.