Question

PROCEDURES
Lit on candle and spirit lamp and placed on the each side of the set up to prevent microorganisms from spreading and divided the plate into five segments and labeled them as sample 1,sample 2,sample3, + control and – control. And then took the viel containing salt solutions were heated over the flame several times before open it. This is to get rid of microorganisms that might contaminate the area, and then dip the swabs into the viel, make sure it completely wet. Then swab the petri dish using the swab while holding the swab plate close to the flame. And the closed the petri dish and heated it several times and dispose the swab. And with the help of forcep, took three pieces of filter papers and placed them on the plate where sample1 (straw berry), sample 2(citrus lemon) and sample 3 (green apple) and other piece on the - control indicated on the plate segments and fleat the forcep before reusing it. Then placed a Chloramphenicol on the + control segment and one drop of deionized water on a – control filter paper. And then, used micropipette to suck in small amount of samples (straw berry, citrus lemon, and green apple) and placed them on the filter papers corresponding to the numbered samples while holding the petri dish closed to the flame. And then closed the petri dish and sealed it with parafilm tape. Then record the initial time and leave it for 24 hours. After 24 hours take note of the zone of inhibition on each segment as shown on the result table.

note: this is the experiment we did last week, correct my tenses and sentences structure without changing the content of the procedures.

Answers

Answered by GPT-4o mini
**PROCEDURES**
A candle and a spirit lamp were lit and placed on each side of the setup to prevent microorganisms from spreading. The plate was divided into five segments and labeled as Sample 1, Sample 2, Sample 3, + Control, and – Control. The vial containing the salt solutions was heated over the flame several times before being opened. This was done to eliminate any microorganisms that might contaminate the area. A swab was dipped into the vial, ensuring it was completely wet. The petri dish was then swabbed using the wet swab while holding it close to the flame. After this, the petri dish was closed, heated several times, and the swab was disposed of.

Using forceps, three pieces of filter paper were taken and placed on the plate where Sample 1 (strawberry), Sample 2 (citrus lemon), and Sample 3 (green apple) were designated, with one piece on the – Control segment. The forceps were flamed before being reused. A drop of chloramphenicol was placed on the + Control segment, and one drop of deionized water was placed on the – Control filter paper.

Next, a micropipette was used to suck in small amounts of the samples (strawberry, citrus lemon, and green apple) and these were placed on the corresponding filter papers according to the labeled samples, while holding the petri dish close to the flame. The petri dish was then closed and sealed with parafilm tape. The initial time was recorded, and the setup was left for 24 hours. After 24 hours, the zone of inhibition on each segment was noted, as shown in the results table.

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