To accurately determine which species is most closely related to species 4 based on a phylogenetic tree, I would need to see the tree diagram itself. However, I can provide some general guidance on how to interpret such trees:
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Common Ancestor: Closely related species share a more recent common ancestor. Look for the most recent branching point (node) that includes species 4 and its relatives.
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Branch Lengths: If branch lengths are used to indicate genetic distance or time of divergence, shorter branches suggest closer relationships.
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Sister taxa: The species that branch off from the same node as species 4 are considered its closest relatives.
Without the phylogenetic tree, I can’t provide a definitive answer. If you can describe the tree or give additional details, I would be glad to help you deduce which species is most closely related to species 4. If no tree images are available, the answer is most likely "1 or 3 (Can't tell)" if it is ambiguous, as the relationships cannot be determined without clear visual representation.