To determine which option contributed most directly to the enactment of a law related to indigenous nations or religious instruction, let's analyze each choice.
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Choice A (indigenous nations’ requests for religious instruction): This directly relates to a law that would be enacted regarding religious instruction for indigenous people, as their requests would be a primary impetus.
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Choice B (religious conflict during the Protestant Reformation): While significant in a broader historical context, this does not directly relate to the enactment of a specific law concerning indigenous nations.
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Choice C (European demands for laborers in the New World): This may influence laws related to labor but not specifically to religious instruction or laws concerning indigenous rights.
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Choice D (epidemic diseases reducing the indigenous population): While this had a profound impact on indigenous populations and their societies, it does not directly relate to the enactment of a law concerning religious instruction.
Given the focus on religious instruction for indigenous peoples, the answer is:
A) indigenous nations’ requests for religious instruction