For Question 1, the answer choice that was NOT indicated by Chargaff's discovery is:
C: When pairing nucleotides, you must pair purines with purines and pyrimidines with pyrimidines.
Chargaff's rules indicate that adenine pairs with thymine and guanine pairs with cytosine, which involves pairing a purine with a pyrimidine, not purines with purines or pyrimidines with pyrimidines.
For Question 2, the answer choice that must also be universal is:
C: The codons in all organisms' DNA code for the same amino acids.
The genetic code (which can be represented by codons) is universal among all known organisms, meaning that the same codons correspond to the same amino acids across all species.