Based on the fossil record data provided, a mass extinction event appears to have occurred between Rock Layer B and Rock Layer C.
This is evident because Rock Layer B contains fossils B1, B2, and B3, while Rock Layer C contains a different set of fossils (C1, C2, D2, E2, E3). Since there is no overlap in the fossil types between these two layers, it suggests that a significant number of species went extinct during this transition, indicative of a mass extinction event.
Therefore, the correct response is: B and C.