Question

Which phospholipase is primarily responsible for generating DAG from phospholipid during signal transduction
GPT-4o mini
The phospholipase primarily responsible for generating diacylglycerol (DAG) from phospholipid during signal transduction is phospholipase C (PLC). When activated, PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) in the cell membrane, resulting in the production of two key signaling molecules: inositol trisphosphate (IP3) and diacylglycerol (DAG). DAG plays a crucial role in various signaling pathways, including the activation of protein kinase C (PKC).