The best response to explain why higher temperatures are not advisable when studying the effect of proteases on proteins is:
A This could result in an irreversible denaturing of the protein.
Higher temperatures can lead to the denaturation of proteins, which is the process where the protein structure unfolds and loses its functional shape. At temperatures above 37°C, many proteins may permanently lose their three-dimensional structure, rendering them inactive and unable to interact properly with proteases.