In the processes of gene expression, the role of transfer RNA (tRNA) is specific to the translation stage. In the context of your description, if we assume the steps represent the following processes:
- Step A could represent DNA transcription to mRNA,
- Step B could represent the processing of mRNA,
- Step C could be the translation of mRNA into a protein,
- Step D could be the final protein or product.
tRNA is essential during the translation phase (Step C), where it carries amino acids to the ribosome to match the mRNA codons and assemble the polypeptide chain.
Thus, the answer would be Step C, where tRNA is necessary.