Question

Original DNA: AAT TTA ACG TAG CTA TGA

(same as last question, copy paste) original mRNA:

(same as last question, copy paste) original Amino Acid sequence (use three letter abbreviation):

Mutation #2 DNA: AAT TTA AGG TAG CTA TGA

Mutation #2 mRNA:

Mutation #2 Amino Acids:

Mutation #2 Type:

Answer 1

To analyze the provided original DNA sequence and the mutated sequence, let's go through each part step by step:

### Original DNA
**Sequence:** AAT TTA ACG TAG CTA TGA

### Original mRNA
To transcribe the DNA to mRNA, we convert each DNA nucleotide to its complementary RNA nucleotide (A → U, T → A, C → G, G → C).

**Original mRNA Sequence:**
```
UUA AAU UGC AUC GAU ACU
```

### Original Amino Acid Sequence
Next, we translate the mRNA sequence into amino acids. The mRNA is read in codons (groups of three nucleotides) to find the corresponding amino acids.

Using a codon table, the translation is as follows:
- UUA → Leu (Leucine)
- AAU → Asn (Asparagine)
- UGC → Cys (Cysteine)
- AUC → Ile (Isoleucine)
- GAU → Asp (Aspartic Acid)
- ACU → Thr (Threonine)

**Original Amino Acid Sequence:**
```
Leu Asn Cys Ile Asp Thr
```

### Mutation #2 DNA
**Sequence:** AAT TTA AGG TAG CTA TGA

### Mutation #2 mRNA
Transcribing the mutated DNA to mRNA, we perform the same complement conversion.

**Mutation #2 mRNA Sequence:**
```
UUA AAU UCC AUC GAU ACU
```

### Mutation #2 Amino Acids
Now we'll translate the mutated mRNA sequence.

Using the codon table again:
- UUA → Leu (Leucine)
- AAU → Asn (Asparagine)
- UCC → Ser (Serine)
- AUC → Ile (Isoleucine)
- GAU → Asp (Aspartic Acid)
- ACU → Thr (Threonine)

**Mutation #2 Amino Acid Sequence:**
```
Leu Asn Ser Ile Asp Thr
```

### Mutation #2 Type
The mutation from the original DNA sequence (where ACG was changed to AGG) can be classified as a missense mutation because it results in a change in the amino acid sequence (Cys changed to Ser).

### Summary
- **Mutation #2 mRNA:** UUA AAU UCC AUC GAU ACU
- **Mutation #2 Amino Acids:** Leu Asn Ser Ile Asp Thr
- **Mutation #2 Type:** Missense Mutation