To identify the two areas that made it possible for people in these ecosystems to use the trans-Saharan trade route, you would focus on:
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Sahel - This region is a transitional zone between the desert and savanna, allowing for access to the desert while supporting agriculture and animal husbandry.
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Savanna - This ecosystem is characterized by grasslands with some trees, providing enough resources for herding and farming, which were vital for trade purposes.
The desert itself would pose challenges for trade, while the rain forest is less conducive to long-distance trade due to dense vegetation and limited transport routes. Therefore, the Sahel and Savanna are the key areas that supported the trans-Saharan trade route.