Why does a microscope slide need to be transparent?

Question

so light can pass through the slide to the objective

so light can partially refract from the slide

so light can spread out evenly along the slide

so light can reflect back onto the light source

Answer 1

A microscope slide needs to be transparent so light can pass through the slide to the objective. This allows the light from the microscope’s illumination source to illuminate the specimen mounted on the slide, enabling clear observation and magnification through the objective lens.