The substance that should be added to the water in order for the bubbles to reappear is carbon dioxide.
Photosynthesis is the process by which plants convert carbon dioxide and water into glucose and oxygen, using sunlight as energy. The bubbles observed on the plant's leaves were likely oxygen produced during photosynthesis. If there is insufficient carbon dioxide in the water, the plant may not be able to perform photosynthesis effectively, resulting in no bubbles. Adding carbon dioxide would help restore the photosynthesis process, leading to the production of oxygen bubbles.