To determine when there was more variation in the spot size of guppies in the river, we need to analyze the histograms.
Looking at the histogram titled "40 Years Ago," the data shows a high concentration of guppies with spot sizes between 5 and 10, which indicates a more narrow range of variation compared to the entire potential range (1 to 10).
In contrast, the histogram titled "Last Year" displays a more balanced distribution, peaking at spot sizes 5 and 6, and including more diverse spot sizes (3 to 8) while having no samples for sizes 1, 2, 9, and 10.
The presence of bars across a broader range (with some sizes missing but still showing a wider span of represented sizes) in the last year suggests there is greater variation in spot sizes.
Given this analysis, the correct response would be:
d. There was more variation last year.