The most likely scenario among the options provided is:
The barrier will initially geographically isolate all of the animals in the forest. Over time, each animal species will experience mutation, resulting in new species of all animals in the forest.
This option accurately reflects the consequences of constructing a barrier in a forested area. When a road with barriers is built, it can physically separate animal populations, limiting their ability to interbreed. This geographical isolation can lead to different selective pressures on the separate populations, which may result in various mutations over time. These mutations, combined with natural selection, can ultimately lead to the emergence of new species as the populations adapt to their distinct environments.