Question
Once you have stained the finished gel mold and put it in the UV light, you can see the bands of DNA sorted by length. The longer the length, the more base pairs a DNA strand contains, and the shorter the length, the fewer base pairs it contains. How did the DNA get sorted from closest to furthest from the wells?
Question 10 options:
Longest strands stayed closer to the wells, shorter strands were further
Shorter strands stayed closer to the wells, longer strands were further
There was no measurable difference in strand placement
Unable to determine using gel electrophoresis
Question 10 options:
Longest strands stayed closer to the wells, shorter strands were further
Shorter strands stayed closer to the wells, longer strands were further
There was no measurable difference in strand placement
Unable to determine using gel electrophoresis
Answers
GPT-4o mini
The correct option is:
**Shorter strands stayed closer to the wells, longer strands were further.**
In gel electrophoresis, DNA fragments are separated based on their size. When an electric current is applied to the gel, the negatively charged DNA fragments move towards the positive electrode. Shorter DNA strands can move through the gel matrix more easily than longer strands, resulting in shorter strands traveling further away from the wells, while longer strands stay closer to the wells.
**Shorter strands stayed closer to the wells, longer strands were further.**
In gel electrophoresis, DNA fragments are separated based on their size. When an electric current is applied to the gel, the negatively charged DNA fragments move towards the positive electrode. Shorter DNA strands can move through the gel matrix more easily than longer strands, resulting in shorter strands traveling further away from the wells, while longer strands stay closer to the wells.