If a gene pool of two populations are separated the populations may develop into new species this is called

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This process is called "speciation." Specifically, when two populations of the same species are separated by geographical barriers (such as mountains, rivers, or distances), they may undergo genetic divergence over time due to different environmental pressures, mutations, and natural selection. Eventually, these changes can lead to reproductive isolation, resulting in the formation of new species. This type of speciation is often referred to as "allopatric speciation."