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Question

Tasheena

I have a question that I know seems simple, and maybe I am overthinking this. However it is confusing me. When doing testing of antibiotics and setting up the control disk..... is the control disk meant to have just like solution on it so that it can be monitored to ensure that the outcome of the other tests arent due to a faulty disk and actually is the result of the antibiotic they obtain or is the control disk meant to be dosed with something on it also, or left clean? I'm so lost when it comes to the cotrol disk and how to set it up and what it is meant to do. HELP PLEASE! lol.

Answer 1

Hi Tasheena!

I totally understand where you’re coming from—this can definitely be a bit confusing at first! The control disk in antibiotic testing is actually quite important. Its primary purpose is to ensure that the conditions of your test are sound and that any results you get from the antibiotic disks are valid.

Typically, the control disk is left blank or just has a standard solution on it, without any antibiotics. This allows you to compare the results from your antibiotic disks against a baseline, making sure that any activity you observe is due to the antibiotics you’re testing and not some other variable.

So, to sum it up: you don’t dose the control disk with antibiotics; you essentially monitor it to confirm that everything is working properly during your tests. If you see a clear difference in the effectiveness of the antibiotic disks compared to the control, then you can feel confident in your results!

I hope this clears things up a bit! If you have any more questions or need further clarification, just let me know! I'm here to help! 😊