The key difference between DNA and RNA among the options provided is:
C: DNA contains thymine, while RNA contains uracil.
This statement accurately describes one of the fundamental differences between the two nucleic acids.
To clarify the inaccuracies in the other options:
A: DNA is typically found in the nucleus, while RNA is found in the cytoplasm. (This is the correct structure, but it’s the opposite of what you stated.)
B: DNA is double-stranded, while RNA is typically single-stranded. (This statement is misleading as DNA should be described as double-stranded, and RNA single-stranded instead of using "while DNA".)
D: DNA uses deoxyribose sugar, while RNA uses ribose sugar. (This is also a reversal; DNA has deoxyribose, and RNA has ribose.)
Thus, option C is the correct choice.