In sentence 25, the word "However" introduces a contrast to the previous statement regarding scientists not being sure about why sleep is essential for many animals. The best transition to maintain coherence and provide a logical flow would be "In contrast" (option J). This is because it emphasizes the difference between the uncertainty of the necessity of sleep for every species and the certainty that some species do require sleep.
So, the most effective transition to replace "However" in sentence 25 is J: In contrast.