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Athens was defeated during the peloponnesian war by an alliance led by the

Macedonians
Persians
Spartans
Delian league

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Athens was defeated during the Peloponnesian War by an alliance led by the Spartans. The war was fought between Athens and its empire (the Delian League) and Sparta and its allies (the Peloponnesian League) from 431 to 404 BCE.