If the solvent is allowed to reach the top, then the separated spots continue moving to the top and eventually would recombine at the top and you would not have separated the spot into the different dompounds.
Larger sample spots may smear as the compounds separate.
Concerning TLC plates...
1. why should't I allow the solvent to reach the top of the plate?
(I assume it's the same as using paper like I did in gen chem but I'm not sure I know why it shouldn't be allowed to reach the top... what would happen?)
2. why sholdn't the spot sample placed on the plate be too large?
~my thought is that we would get mixed up in where the center of the spot is since it's too large or it could get mixed with the compound placed next to it if too large but I'm not sure.
If anyone wants to help me out it would be great..
~Thanks~
2 answers
Thanks Dr.Bob =)
1 answer