To derive the mRNA sequence from the given DNA sequence, you will need to perform transcription, which involves replacing each DNA base with its complementary RNA base. In DNA, adenine (A) pairs with uracil (U) in RNA, thymine (T) pairs with adenine (A), cytosine (C) pairs with guanine (G), and guanine (G) pairs with cytosine (C).
Given DNA Sequence:
DNA: T A C G A C C T T G G C G A C G A C T
Transcription to mRNA Sequence:
mRNA: A U G C U G G A A C C G C U G C U G A
(Note: The bases have been transcribed according to the complementary base pairing: T → A, A → U, C → G, G → C.)
Amino Acid Sequence:
To determine the amino acid sequence, we will translate the mRNA sequence using the standard genetic code. The mRNA is translated in codons, which are groups of three nucleotides.
- A U G → Methionine (Met)
- C U G → Leucine (Leu)
- G A A → Glutamic acid (Glu)
- C G C → Arginine (Arg)
- U G C → Cysteine (Cys)
- U G A → Stop codon
Result:
mRNA Sequence: A U G C U G G A A C C G C U G C U G A
Amino Acid Sequence: Met-Leu-Glu-Arg-Cys
Will there likely be effects?
To determine whether there will likely be effects from this mutation, we need more information about the specific change in the DNA sequence compared to the original. If you provide the original DNA sequence, we can identify whether the mutation resulted in a change in the amino acid sequence or an altered function of the protein.
Type of Mutation:
From the data presented, without knowing the original DNA sequence to directly compare with, we can't definitively classify the type of mutation. However:
- If a base is changed and it alters the resulting amino acid, it's usually categorized as a missense mutation.
- If a base is changed and leads to a premature stop codon, it's a nonsense mutation.
- If there are insertions or deletions, these can result in frameshift mutations.
For a precise classification, the original DNA sequence is required. Please provide the original sequence so we can evaluate these aspects further!