To determine the mRNA sequence, we first need to transcribe the given DNA sequence. The transcription process involves replacing thymine (T) in the DNA with uracil (U) in the mRNA. The given DNA sequence is:
DNA Sequence: T A C A T C T T G G C G A C G A C T
Let's transcribe it to mRNA:
mRNA Sequence: A U G U A G A A C C G C U G C U G A
Next, we will encode the mRNA into the corresponding amino acid sequence using the genetic code, where each set of three nucleotides (codon) encodes one amino acid:
| Codon | Amino Acid | |---------|------------------| | A U G | Methionine (Met) | | U A G | Stop | | A A C | Asparagine (Asn) | | C G C | Arginine (Arg) | | U G C | Cysteine (Cys) | | U G A | Stop |
So the resulting amino acid sequence from the mRNA would be:
Amino Acid Sequence: Met - Stop (only one amino acid before a stop codon)
Effects & Mutation Type
As for the effects, whether there are likely to be any effects depends on the position of the mutation and its nature. In this case, the mutation results in a premature stop codon (UAG).
- Type of mutation: This appears to be a nonsense mutation, where a single nucleotide change leads to a premature stop codon.
This type of mutation can have significant effects, potentially leading to a nonfunctional protein if the stop codon occurs early in the sequence. Therefore, it's likely there would be effects on protein function due to a truncated protein product.
In summary:
- mRNA Sequence: A U G U A G A A C C G C U G C U G A
- Amino Acid Sequence: Met - Stop
- Mutation Type: Nonsense mutation
- Likely Effects: Yes, significant effects due to a truncated protein.