To determine which two individuals have the trait in the pedigree you mentioned, we need to look for the darkened figures representing those who express the trait. Since I cannot see the image, I can't provide a specific answer tailored to the pedigree you've described.
However, you can analyze the options based on the individuals shown in your pedigree:
- A. Two sisters: Look for two darkened circles that represent females.
- B. The father and the mother: Check if both individuals (a box and a circle) are darkened.
- C. The grandfather and a granddaughter: Look for a darkened box (the grandfather) and a darkened circle (the granddaughter).
- D. The grandmother and a grandson: Check for a darkened circle (the grandmother) and a darkened box (the grandson).
Whichever option corresponds to the individuals that are darkened in the pedigree indicates who has the trait.