Review: DNA is found in three unique locations in an insect cell that is infected with

In an insect cell infected with Wolbachia, the three unique locations of DNA would include:

1. Mitochondrial DNA: As you've already mentioned, DNA is found in the matrix of the mitochondria. In insects, mitochondria possess their own circular DNA, which is distinct from nuclear DNA.

2. Nuclear DNA: The primary DNA in insect cells is found in the nucleus. In this case, the insect's genomic DNA is housed here, which contains the genetic instructions for the insect’s biological functions.

3. Wolbachia DNA: Wolbachia are intracellular bacteria that often infect insect cells. They have their own circular DNA located within their cells, typically found in the cytoplasm of the host cell.

To see these locations in an electron micrograph (EM) of an insect cell infected with Wolbachia, you would look for:

• Mitochondria: These will appear as double-membraned organelles with inner structures (cristae) visible in the EM.
• Nucleus: This will be identifiable as a large, membrane-bound structure with a darker area indicating the chromatin.
• Wolbachia: These may appear as inclusions or small, oval structures within the cytoplasm of the insect cell, often near or associated with the mitochondria.

Overall, in an annotated EM, you'd find the mitochondria filled with DNA, the nucleus containing chromatin, and the Wolbachia along with their plasmids or genomic DNA dispersed throughout the cytoplasm.