Suppose that a portion of double-stranded DNA in the middle of a large gene is being transcribed by an RNA polymerase. As the polymerase moves through the sequence of six bases shown in the diagram below, what is the corresponding sequence of bases in the RNA that is produced?

Question

A portion of a double-stranded DNA. CCGAGT are located on the coding strand, from the 3 prime end to the 5 prime end. GGCTCA are located on the template strand from the 5 prime end to the 3 prime end.

Enter the sequence of bases as capital letters with no spaces and no punctuation. Begin with the first base added to the growing RNA strand, and end with the last base added.

Answer 1

To determine the RNA sequence that is produced from the given portion of double-stranded DNA, we need to understand the process of transcription.

In transcription, RNA polymerase synthesizes RNA using the template strand of DNA. The RNA sequence is complementary to the template strand and has uracil (U) instead of thymine (T).

Given the template strand sequence (5' to 3'): GGCTCA The corresponding RNA sequence (which is synthesized in the 5' to 3' direction) would be complementary base pairing:

• G pairs with C
• G pairs with C
• C pairs with G
• T pairs with A
• C pairs with G
• A pairs with U

Thus, the RNA sequence synthesized from this template strand will be:

5' - CCGAGU - 3'

So, the corresponding sequence of bases in the RNA that is produced is:

CCGAGU

In capital letters with no spaces or punctuation, the answer is:

CCGAGU