Before a molecule of mRNA can be translated into a protein on the ribosome, the mRNA must first be transcribed from a sequence of DNA. Stages of protein biosynthesis. The first step is transcription of mRNA from the DNA template strand. The second step is synthesis of a protein from the mRNA, when each codon complies with one amino acid. The DNA template strand is arranged from the 5 prime to the 3 prime end and mRNA is arranged vice versa, so does the other DNA strand. DNA consists of A, T, G, and C, while mRNA has U instead of T. What amino acid sequence does the following DNA nucleotide sequence specify?

To determine the amino acid sequence specified by the provided DNA nucleotide sequence, we first need to transcribe the DNA into mRNA and then translate the mRNA into an amino acid sequence.

### Step 1: Transcription of DNA to mRNA

The given DNA sequence is:
3′−TAC AGA ACG GTA−5′

Since mRNA is synthesized from the DNA template strand in the 5' to 3' direction, we need to find the complementary sequence of bases. Here’s the matching based on base pairing (A with U, T with A, C with G, and G with C):

This means we take the complementary strand in the 5' to 3' direction:
5'−AUG UCU UGC CAU−3' (this is the mRNA strand)

### Step 2: Translation of mRNA to Amino Acids

Now we will translate the mRNA sequence into an amino acid sequence using the genetic code.

The mRNA sequence is:
5'−AUG UCU UGC CAU−3'

We can break that down into codons (three-nucleotide sequences):
- AUG
- UCU
- UGC
- CAU

Now we will translate each codon into its corresponding amino acid using standard codon table:

1. **AUG** - Methionine (Met)
2. **UCU** - Serine (Ser)
3. **UGC** - Cysteine (Cys)
4. **CAU** - Histidine (His)

### Final Amino Acid Sequence
Putting these together using three-letter abbreviations, the amino acid sequence is:

**Met-Ser-Cys-His**