The correct response to highlight a difference between gene regulation in prokaryotes and eukaryotes is: Prokaryotes do not store their genes in a nucleus like eukaryotes do.
This statement accurately reflects one of the fundamental structural differences between prokaryotic and eukaryotic cells, which has implications for gene regulation. In prokaryotes, the DNA is located in the cytoplasm and is not contained within a nuclear membrane, whereas in eukaryotes, the DNA is housed within a membrane-bound nucleus.