To identify which sequence shows a substitution mutation, we need to compare each of the given sequences to the original sequence:
Original Sequence: C G T T A C T G C A A T G C A A T G A C G T T A
Now let's look at each option:
-
CGTCTACTGCAAT GCAGATGACGTTA
- Comparison: C G T T C T A C T G C A A T G C A G A T G A C G T T A
- Changes: "T" at the 3rd position is substituted with "C" and "C" at the 13th position is substituted with "G." (2 substitutions)
-
CGTTACTGCAAT GCAATGACGTTA
- Comparison: C G T T T A C T G C A A T G C A A T G A C G T T A
- Changes: No changes detected.
-
CGTGACTGCAAT GCACTGACGTTA
- Comparison: C G T G A C T G C A A T G C A C T G A C G T T A
- Changes: "T" at the 3rd position is substituted with "G." (1 substitution)
-
CGTACTGCAAT GCATGACGTTA
- Comparison: C G T A C T G C A A T G C A A T G A C G T T A
- Changes: "T" at the 3rd position is substituted with "A." (1 substitution)
The sequences that show a substitution mutation are sequences 3 and 4. However, sequence 1 has multiple substitutions.
Thus, the sequences that show a substitution mutation are clearly:
- CGTCTACTGCAAT GCAGATGACGTTA (multiple substitutions)
- CGTGACTGCAAT GCACTGACGTTA (single substitution)
- CGTACTGCAAT GCATGACGTTA (single substitution)
If the question asks for only one option, then the first option shows clear substitution mutations.