In gel electrophoresis, the migration of DNA fragments, including PCR products, is influenced by their size. Generally, smaller fragments travel faster and further through the gel matrix compared to larger fragments.
Given this context:
a. Which PCR product is closest to the wells?
The 16S rRNA bands would typically be closest to the wells because they are larger in size compared to CO1.
b. Which PCR product traveled furthest through the gel?
The CO1 bands would have traveled furthest through the gel, as they are usually smaller than the 16S rRNA bands.
c. Which is the largest amplified product?
The 16S rRNA is generally the largest amplified product compared to CO1.
Keep in mind that the actual sizes can vary depending on the specific primers and the organisms being amplified, but this is the typical behavior in gel electrophoresis.